ABSTRACT
This paper is in order to study the anti-feeding and growth inhibition activity of toatal ginsenoside of ginseng stems and leaves against 4th-instar Mythimna separata larvae. Simulating natural growing condition indoors, on the base, To study the anti-feeding and growth inhibition activity of toatal ginsenoside against 4th-instar M. separata larvae by leaf disc test. The toatal ginsenoside appeared to be of significant antifeeding activity against 4th-instar M. separata larvae. The 4th-instar M. separata larvae fed on the leaves of Sorghum bicolor treated with 20, 10, 5 g · L(-1) toatal ginsenoside. At 8 h, non-selective anti-feeding rate were 88.67%, 64.40% and 47.36%, and selective anti-feeding rate were 62.49% , 44.29% and 34.19%; Compared with the photographic, The toatal ginsenoside conld make the development period had prolonged 13h in treated group. The toatal ginsenoside had significant inhibition effect on feeding and growth and development against 4th-instar M. separata larvae, and inhibition effect increases as the increase of concentration ginsenoside.
Subject(s)
Animals , Ginsenosides , Pharmacology , Insecticides , Pharmacology , Larva , Moths , Panax , ChemistryABSTRACT
Under indoors simulating natural growing condition, the 4th-instar Mythimna separata larvae were fed by using poi- son leaf disk method. The effect of total ginsenosides on the protective enzymes (PPO, T-SOD, CAT and POD) of M. separata larvae was studied. The total ginsenosides could influence the protective enzymes of 4th-instar M. separata larvae significantly. After treated by total ginsenosides, the PPO activities increased firstly then decreased, and tended to equilibrium, and reached the maximum after 48 h. Furthermore, the total ginsenosides disturbed the dynamic balance of SOD, CAT and POD of M. separata larvae, and the yield of O2-* speeded. The results suggest that the total ginsenosides influence the protective enzymes of 4th-instar M. separata larvae, and disturb the original dynamic balance of protective enzymes. Consequently the insect suffers from the harm of O2-*.
Subject(s)
Animals , Enzymes , Metabolism , Ginsenosides , Metabolism , Larva , Metabolism , Lepidoptera , Metabolism , Oxygen , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study the anti-feeding effect of total ginsenoside of ginseng stems and leaves on Heliothis dipsacea larvae.</p><p><b>METHOD</b>The natural growing condition for lavae was simulated indoors. The anti-feeding effect of total ginsenoside on Heliothis dipsacea larvae was studied by leaf disc test.</p><p><b>RESULT</b>The total ginsenoside appeared showed a significant antifeeding effect. The Heliothis dipsacea larvae fed with the leaves of soybean treated with 2.0%, 1.0% and 0.5% total ginsenoside, respectively. At 8 h, non-selective anti-feeding rate were 93.40%, 83.42% and 75.19%, and selective anti-feeding rate were 77.53% , 73.58% and 58.86%.</p><p><b>CONCLUSION</b>The toatal ginsenoside had significant inhibition effect on Heliothis dipsacea larvae, and inhibition effect increases as the increase of concentration ginsenoside.</p>
Subject(s)
Animals , Feeding Behavior , Ginsenosides , Pharmacology , Larva , Physiology , Moths , Physiology , Panax , Chemistry , Plant Extracts , Pharmacology , Plant Leaves , Chemistry , Plant Stems , ChemistryABSTRACT
<p><b>OBJECTIVE</b>This study aims to reveal the effect of total ginsenoside on the protein content and digestive enzyme activities of 4th-instar Mythimna separata larvae, including alpha-amylase and cellulose, and explore the ecological function of total ginsenoside.</p><p><b>METHOD</b>While simulating natural growing condition indoors, 4th-instar M. separata larvae were fed by poison leaf disk method. The protein content was tested by Lowry Protein Assay Kit method, the activity of alpha-amylase was measured by dinitrosalicylic acid test, and the activity of cellulase was determined by the filter paper method.</p><p><b>RESULT</b>The total ginsenoside could reduce the content of protein of 4th-instar M. separata larvae significantly, and the activity of digestive enzyme, including alpha-amylase and cellulase. The protein content, alpha-amylase and cellulase activity of treatments were obviously lower than that of the control. Inhibition ratio of alpha-amylase and cellulase activity was positively correlated with total ginsenoside concentration: i. e. 20 g x L(-1) > 10 g x L(-1) > 5 g x L(-1).</p><p><b>CONCLUSION</b>The results suggest that the inhibition effect of total ginsenoside on protein content and digestive enzymes may be one of the causes to antifeedant and dysplasia of M. separata larvae.</p>